Analysis Antioxidant IC50 Liquid Smoke of Cocoa Skin with Several Purification Methods

Cocoa skin waste can be used as the raw material for making liquid smoke by pyrolysis. Liquid smoke has a function as the preservative in food. The use of liquid smoke as the preservative must go through several purification processes. The purpose of this study was to determine the antioxidant content of cocoa skin liquid smoke in several purification methods. The purification process uses several methods in purifying liquid smoke, such as distillation, purification with zeolite, purification by active charcoal and the mixture of zeolite and active charcoal. The results of IC50 antioxidant activity of cocoa skin liquid smoke by distillation, purification with zeolite, purification with active charcoal and combined purification between active charcoal and zeolite obtained IC50 values of 217.55 ppm for the distillation method, 164.15 ppm for purification with active charcoal and zeolite, 143.43 ppm for purification with zeolite and 44.05 ppm for purification with active charcoal. Purification with active charcoal is the best treatment and has a very strong category of Antioxidants compared to the other three treatments.